Fig. 4

PSLipos-L-NAC more effectively improved wound healing in lung epithelial cells via macrophage-mediated way. (A) Schematics of inflammatory BMDMs and MLE-12 co-cultured system. The pro-inflammatory cytokines expression of (B) TNF-α, (C) IL-1β, (D) IL-6 in co-culture system was detected after treating with samples using ELISA (n>3). (E) NO production. (F) and (G) Scratch wound assay was used to assess the wound healing of MLE-12 cells under inflammatory environment. Scale bars, 100 μm. Data are from three independent experiments and presented as the mean ± SD. (ns represented no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001)