Fig. 5

Anti-inflammatory effects of inhaled PSLipos-NAC nanosystems in a bleomycin-induced ALI mouse model. (A) Timeline of experiments. The lung tissue and serums were harvested to assess the expression of pro-inflammatory factors at 24 h after treatment. i.t. represented intratracheal injection and a.i. represented aerosol inhalation. (B) Expression of pro-inflammatory genes in lung tissue. (C) Expression of inflammatory factors in serum. (n ≥ 6 per group, ns represented no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001)