Fig. 4
Safety of the SPN in vitro and in vivo. (a) SPN operation diagram. (b) Viability of AR42J cells after culture with different concentrations of SPN. (c) Photographs of RBC haemolysis after incubation with various solutions for approximately 4 h: [1] H2O [2], NaCl, [3] DMEM [4], RPMI-1640 [5], PBS, and [6] CPPO@PFO. (d) Changes in animal body weight recorded over 30 days (n = 3). (e-h) Serum analysis of AST, ALT, BUN and CRE in mice after SPN i.v. injection. (i) H&E-staining of the pancreas and other organs, including heart, liver, kidney, and lung, from mice 30 days after treatment with SPN. (Data represent means ± SDs, ns indicates no significant difference.)