Fig. 1

The combined effect of JQ1 and icaritin in inhibiting breast cancer cells. (A) MTT assay of 4T1 cells treated with JQ1 and icaritin (n = 6). (B) The combination index of JQ1 and icaritin at different concentration ratios (n = 6). (C) Apoptosis analysis of 4T1 cells treated with JQ1 and icaritin (n = 3). (D) Quantitative analysis of the apoptosis of 4T1 cells treated with different drugs (n = 3). (E) Colony formation assay of 4T1 cells treated with JQ1 and icaritin (n = 3). F and G) Protein expression of c-myc was determined by western blotting analysis (n = 3). All data are presented as the mean ± SD. *p < 0.05, **p < 0.01