Fig. 3

The cellular uptake and antitumor efficacy of the prepared nanoparticles. (A) Confocal imaging of cellular uptake under normoxic and hypoxic conditions at 3 h (n = 3). (B) Flow cytometry of cellular uptake under normoxic and hypoxic conditions at 3 h (n = 3). (C) MTT assay of prepared nanoparticles under normoxic conditions (n = 5). (D) MTT assay of prepared nanoparticles under hypoxic conditions (n = 5). (E) Colony formation assays of prepared nanoparticles under normoxic or hypoxic conditions (n = 3). (F) Flow cytometry assays show apoptosis of prepared nanoparticles under normoxic or hypoxic conditions (n = 3). (G) Analysis of apoptosis of prepared nanoparticles under normoxic or hypoxic conditions (n = 3). All data are presented as the mean ± SD. *p < 0.05, **p < 0.01