Fig. 3

A Viabilities of osteoblast cells (MC3T3) cultured with different materials; B Fluorescence images of osteoblast cells (MC3T3) stained with Calcein AM (green, live cells) and PI (red, dead cells); C SEM images of MC3T3 cells co-cultured with BTZ/nHA@SA scaffold at day 3; D Representative images of ALP staining and ARS staining of MC3T3 cells cultured with nHA@SA and BTZ/nHA@SA extract at day 7 or day 14; E Relative mRNA expression levels of osteogenic genes Alp at day 3, 5 and 7; F Relative mRNA expression levels of osteogenic genes Sp7 at day 3, 5 and 7; G Expression levels of RUNX2 and COL1 in MC3T3 cells after 7 days; H Viabilities of breast cancer cells (4T1) cultured with different materials; I Fluorescence images of breast cancer cells (4T1) stained with Calcein AM (green, live cells) and PI (red, dead cells). *p < 0.05, **p < 0.01, ***p < 0.001