Fig. 2

MSC-ex diminishes LOXL2 expression derived from activated HSC. A Immunofluorescence staining of exosomal marker CD9 (Red) and HSC activation marker α-SMA (Green) in PBS or MSC-ex treated mouse livers. Scale bars, 20 μm. B Western blot analysis and quantification of α-SMA in PBS or MSC-ex treated mouse livers (n = 3; **p < .01). C Representative fluorescent images showed the uptake of PKH67-labeled MSC-ex (PKH67-ex) by LX-2 cells. Scale bars, 20 μm. D qRT-PCR analysis for LOXL2 and α-SMA mRNA in PBS, MSC-ex, or BAPN treated LX-2 cells (n = 4; **p < .01, ***p < .001, and ^###p < .001). E Immunofluorescence staining images of LOXL2 and α-SMA protein in PBS, MSC-ex (200 μg/mL), or BAPN (1.0 mg/mL) treated LX-2 cells. Scale bars, 50 μm. F Western blot analysis and quantification of LOXL2 and α-SMA protein in PBS, MSC-ex (200 μg/mL) or BAPN (1.0 mg/mL) treated LX-2 cells (n = 4; ***p < .001, ^###p < .001)