Fig. 4

IdentifyingLOXL2 as a downstream target gene of YAP in LX-2. A Western blot analysis and quantification of YAP and LOXL2 protein expression in PBS and TGFβ activated LX-2 cells (n = 4; **p < .01, ^###p < .001). B Immunofluorescence staining images of YAP (Red) and LOXL2 (Green) protein in PBS and TGFβ activated LX-2 cells. Scale bars, 50 μm. C qRT-PCR analysis for LOXL2 mRNA in Ad-GFP, Ad-YAP, sh-Ctr, or transfected LX-2 cells (n = 4; ***p < .001). D Western blot analysis and quantification of YAP and LOXL2 protein expression in sh-Ctr or sh-YAP transfected LX-2 cells (n = 4; ***p < .001, ^##p < .01, and n.s., Not significant). E Western blot analysis and quantification of YAP and LOXL2 protein expression in Ad-GFP or Ad-YAP transfected 293T, HL7702, and LX-2 cells (n = 4; ***p < .001, and ^##p < .01, and ^###p < .001)