Fig. 5

miR-27b-3p enriched in MSC-ex and downregulated YAP expression. A Relative percentage of miRNAs in total miRNA reads. B The predicted binding site of miR-27b-3p targeting the 3ʹ-UTR of YAP. C qRT-PCR of miR-27b-3p in isolated MSC and MSC-ex (n = 3). D FISH analysis of U6 and miR-27b-3p in PBS or MSC-ex treated LX-2 cells. Scale bars, 20 µm. E Luciferase reporter assay showed YAP as a target of miR-27b-3p (n = 4; **p < .01, n.s., Not significant). NC, negative control; WT, wide type; MUT, mutant. F qRT-PCR of miR-27b-3p in miR-27b-3p mimics transfected LX-2 (n = 6; ***p < .001, n.s., Not significant). G qRT-PCR of YAP mRNA in miR-27b-3p mimics transfected LX-2 (n = 6; **p < .01, ***p < .001). H Western blot and quantification of YAP protein in miR-27b-3p mimic transfected LX-2 (n = 3; *p < .05). I qRT-PCR of miR-27b-3p in miR-27b-3p inhibitors transfected LX-2 (n = 6; ***p < .001). J qRT-PCR of YAP mRNA in miR-27b-3p inhibitors transfected LX-2 (n = 6; ***p < .001). K Western blot and quantification of YAP protein in miR-27b-3p inhibitors transfected LX-2 (n = 3; **p < .01, ***p < .001)