Fig. 6

MSC-ex increased miR-27b-3p expression and downregulated YAP/LOXL2 expression. A qRT-PCR analysis of miR-27b-3p in PBS or MSC-ex (100 or 200 μg/mL) treated LX-2 cells (n = 6; ***p < .001). B qRT-PCR analysis of YAP mRNA in PBS or MSC-ex (100 or 200 μg/mL) treated LX-2 cells (n = 6; ***p < .001). C Western blot analysis and quantification of YAP protein in PBS or MSC-ex (100 or 200 μg/mL) treated LX-2 cells (n = 3; ***p < .001, ^#p < .05, and ^##p < .01). D Immunofluorescence staining images of YAP (Red) and LOXL2 (Green) protein in PBS or MSC-ex treated LX-2 cells. Scale bars, 20 μm. E FISH analysis of U6 and miR-27b-3p in PBS or MSC-ex treated fibrotic livers. Scale bars, 10 µm. F qRT-PCR analysis of miR-27b-3p in PBS or MSC-ex treated fibrotic livers (n = 6; **p < .01). G qRT-PCR analysis of YAP mRNA in PBS or MSC-ex treated fibrotic livers (n = 6; ***p < .001). H Immunohistochemistry staining for YAP, LOXL2, and α-SMA in PBS or MSC-ex treated fibrotic livers. Scale bars, 50 μm