Fig. 8
miR-27b-3p overexpression enhanced YAP/LOXL2 inhibition efficacy of MSC-ex. A qRT-PCR of miR-27b-3p in exosomes isolated from negative control (NC) mimics overexpressed MSC-ex (NCoe-ex) or miR-27b-3p mimics overexpressed MSC-ex (miR-27boe-ex) (n = 6; ***p < .001). B FISH of miR-27b-3p in PBS, 50 μg/mL NCoe-ex or miR-27boe-ex treated LX-2. Scale bars, 20 µm. C–F qRT-PCR of YAP, LOXL2, α-SMA and Col1A2 mRNA in PBS, 50 μg/mL NCoe-ex or miR-27boe-ex treated LX-2 (n = 6; *p < .05, **p < .01, and ***p < .001). G Western blot analysis and quantification of YAP, LOXL2, α-SMA and Col1A2 protein in PBS, NCoe-ex or miR-27boe-ex treated LX-2 (n = 4; *p < .05, ###p < .001, @@p < .01, and &p < .05)