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Table 1 Primers for real-time quantitative PCR

From: Mesenchymal stem cell-derived exosomal miR-27b-3p alleviates liver fibrosis via downregulating YAP/LOXL2 pathway

Genes

Primer sequence (5′-3′)

Annealing temperature (°C)

Product size (bp)

Human LOXL2

For: CTGCAAGTTCAATGCCGAGT

60

149

Rev: TCTCCACCAGCACCTCCACTC

Human Col1A2

For: CTACTGGTGCCAGAGGACTT

58

138

Rev: TAGGGCCTCTCTTTCCTTCT

Mouse LOXL2

For: TTCTGCCTGGAGGACACTGAGT

58

139

Rev: TTCTGCCTGGAGGACACTGAGT

Mouse/Human β-actin

For: CACGAAACTACCTTCAACTCC

56

265

Rev: CATACTCCTGCTTGCTGATC

LOXL2 ChIP

For: GGTTTGTCTCCTCAGGGAGTG

57

102

Rev: GCGAGCTGCAAAACAAGGGA

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Journal of Nanobiotechnology

ISSN: 1477-3155

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