Fig. 5

miR-150-5p is enriched within Sca-1⁺ exosomes and regulates microglial polarization via targeting MEKK3, the mouse analogue of mitogen-activated protein kinase kinase kinase 3 (MAP3K3). A Volcano plot showing miRNAs that are up- (red) and down (blue)-regulated in BM Sca-1⁺ exosomes, compared to Sca-1⁻ ones, as determined by high throughput sequencing. B Heatmap showing the 27 differentially-expressed miRNAs between the 2 groups, with up to twofold change in expression, of which 11 were up-regulated (warm colors, orange-red) and 16 down-regulated (cool colors, light–dark blue) in Sca-1⁺ versus Sca-1⁻ exosomes. C Relative abundance of the 27 miRNAs, in terms of read count, between the 2 groups. D Venn diagram demonstrating that the 27 differentially-expressed miRNA identified in our analysis were also those predicted by different databases. E Gene ontology (GO) enrichment analysis of the target genes for the 27 differentially-expressed miRNAs, in which they fell into 1 of 3 broad categories: biological process (BP), cellular component (CC), and molecular function (MF). F Kyoto genes and genomes (KEGG) enrichment analysis, in the form of a scatter plot, showing pathway associations for the target genes of these 27 miRNAs. G Predicted 3’-UTR binding site for miR-150-5p on the MEKK3 (MAP3K3) gene by TargetScan