Fig. 7

Increased miR-150-5p levels within Sca-1⁺ exosomes downregulate MEKK3/JNK signaling within post-I/R retinas in vivo. Relative expression of miR-150-5p (A) and MEKK3 (B) within the retina among the 4 treatment groups: Normal control, I/R, as well as Sca-1⁺ (I/R + Sca-1⁺ exo) or Sca-1⁻ (I/R + Sca-1⁻ exo) exosomes administered after I/R injury, as determined by RT-qPCR. Representative Western blot images (C) and quantification of MEKK3 (D), p-JNK/JNK (E), and p–c-Jun/c-Jun (F) protein expression levels among the 4 treatment groups, normalized to GAPDH. Data shown as mean ± SEM. n = 3/group for all experiments. **P < 0.01, *P < 0.05