Fig. 7

Antimetastatic activity of nanoparticles in vivo. (A) Number of lung metastasis nodules from 4T1 tumor-bearing mice in each treatment group. (B) H&E staining of tumor and lung tissue sections. Arrows point to the area of intrapulmonary metastasis of tumor cells. Scale bar = 100 μm. (C) Analysis of macrophages in 4T1 tumors by flow cytometry. Total macrophages proportion (a) and M1/M2 ratios (b) of saline and SDPN group-treated tumor tissues. (D) Immunohistochemical staining of IL-10 and IL-6 in tumor tissue sections of saline and SDPN groups. Scale bar = 100 μm. (E-H) The relative mRNA expression of the macrophage-associated markers IL-10, IL-6, CD206, and CD86 in tumors by qPCR assay. (I) Immunofluorescence staining of tumor CD86 and CD206 in tissue sections. Scale bar = 200 μm. Mean ± SD, n = 3; *P < 0.05, **P < 0.01. Abbreviations: SDPN, sophorolipid-associated membrane-biomimetic choline phosphate-poly(lactic-co-glycolic) acid hybrid nanoparticle; qPCR, quantitative polymerase chain reaction; SD, standard deviation; H&E, hematoxylin and eosin