Table 1 Primer sequences of the genes used for quantitative real-time polymerase chain reaction
Gene | Forward primer (5’->3’) | Reverse primer (5’->3’) |
|---|---|---|
TGF-β1 | CTTCAATACGTCAGACATTCGGG | GTAACGCCAGGAATTGTTGCTA |
CCL2 | TAAAAACCTGGATCGGAACCAAA | GCATTAGCTTCAGATTTACGGGT |
TNF-α | CTGAACTTCGGGGTGATCGG | GGCTTGTCACTCGAATTTTGAGA |
I NOS | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGTCAC |
CD206 | CTCTGTTCAGCTATTGGACGC | TGGCACTCCCAAACATAATTTGA |
CD86 | CTGGACTCTACGACTTCACAATG | AGTTGGCGATCACTGACAGTT |
STAT 3 | CACCTTGGATTGAGAGTCAAGAC | AGGAATCGGCTATATTGCTGGT |
HIF1-α | GATGACGGCGACATGGTTTAC | CTCACTGGGCCATTTCTGTGT |
c-Myc | CCCTATTTCATCTGCGACGAG | GAGAAGGACGTAGCGACCG |
LDHA | CAAAGACTACTGTGTAACTGCGA | TGGACTGTACTTGACAATGTTGG |
GLUT1 | TCAAACATGGAACCACCGCTA | AAGAGGCCGACAGAGAAGGAA |
IL-6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
IL-10 | CTTACTGACTGGCATGAGGATCA | GCAGCTCTAGGAGCATGTGG |
GAPDH | AAGAAGGTGGTGAAGCAGG | GAAGGTGGAAGAGTGGGAGT |