Fig. 3

In vitro antitumor effects of 2-BP/CPT-PLNs. A-C In vitro cytotoxicity of 2-BP/CPT-PLNs against B16-F10 cells, 4T1 cells, and NIH-3T3 cells (n = 6). D Mean fluorescence intensity (MFI) was measured by flow cytometry (FCM) to evaluate cellular uptake of 2-BP/CPT-PLNs by B16-F10 cells (n = 3). E, F Cell cycle arrest and cell apoptosis induced by 2-BP/CPT-PLNs were analyzed by FCM (n = 3). G Cell apoptosis within B16-F10 multicellular spheroids induced by 2-BP/CPT-PLNs was evaluated by CLSM. The Bar: 100 μm. Data were represented as mean ± SD. *p < 0.05 or **p < 0.01 represented significance