Fig. 4

The ability evaluation of ICD induced by 2-BP/CPT-PLNs. A-D CRT exposure, HMGB1, ATP release, and upregulation of intracellular ROS level induced by 2-BP/CPT-PLNs against B16-F10 cells were analyzed by FCM, and measured by microplates after being treated with the corresponding ELISA test kits (n = 3). E CRT expression of B16-F10 cells after chemotherapy was evaluated by CLSM. Bar: 20 μm. F The intracellular ROS level of B16-F10 cells after being stained with DCFH-DA was evaluated by CLSM. Bar: 50 μm. G The down-regulation of membrane PD-L1 level in B16-F10 cells induced by 2-BP/CPT-PLNs. H Total PD-L1 protein levels of B16-F10 cells after various treatments were evaluated by WB assay (n = 3). I Immunofluorescence staining of PD-L1 in B16-F10 cells with various treatments. Bar: 10 μm. Data were represented as mean ± SD. *p < 0.05 or **p < 0.01 represented significance