Fig. 5

Tumor growth inhibition and immune response induced by chemo-immunotherapy with 2-BP/CPT-PLNs in vivo. A, B Tumor growth files and body weight changes of B16-F10 tumor-bearing mice during the chemo-immunotherapy. The red arrows represented the time points of every intravenous administration. C, D The morphology and weights of excised tumors from mice after the first treatments for 10 days. E-G Quantification analysis of CD4⁺, CD8⁺, and CD8⁺GzmB⁺ T cells (gated from CD3⁺ T cells) infiltration within tumors. H Relative membrane PD-L1 expression level of tumor cells (gated from CD45^- cells) within tumor tissues. I-J Quantification analysis of CD4⁺, CD8⁺ T cells in spleens from the above-administrated mice. K H&E staining and immunofluorescence staining of Ki67, Tunel, PD-L1, and CD8 of tumor slides. Bar: 50 μm. Data were represented as mean ± SD (n = 7 in A-D, and n = 5 in E-J). *p < 0.05, **p < 0.01 or ***p < 0.001 represented significance