Fig. 3

hucMSC-Ex repairs IBD by inhibiting ferroptosis in the colon of mice. A, Analysis of mRNA expression level of ferroptosis-related genes in mice colon by qRT-PCR; B, Western blot analysis of the expression level of ferroptosis-related molecules in colon tissue of mice; C, TUNEL staining of colon tissues of mice (scale bar = 100 μm); D, Prussian blue staining of colon tissues of mice (scale bar = 50 μm); E, IHC analysis of colon tissue level of ACSL4 and the average optical density (scale bar = 50 μm); F, Total GSH content in the colon of mice. Data are presented as mean ± SD by student t-test. *P < 0.05; **P < 0.01; ***P < 0.001.