Fig. 8

hucMSC-Ex transfected with miR-129-5p targets ACSL4 to inhibit ferroptosis in DSS-induced IBD mice intestinal epithelial cells. A, The expression level of ferroptosis-related molecular in mice intestinal tissue; B, T-GSH concentration in mice colon tissue; C, The expression level of ferroptosis-related molecules in mice colon by western blot; D, TUNEL staining (scale bar = 100 μm); E, Prussian blue staining of mice intestinal tissue (scale bar = 50 μm); F, The expression of ACSL4 as detected by immunohistochemistry and the average optical density (scale bar = 50 μm). Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001