Fig. 3
In vitro cellular uptake triggered by the DNA nanostructure-based drugs codelivery system. (A) Confocal images and (B) flow cytometry analysis of 4T1 cells incubated for 6 h with three different conditions: DNA origami nanovehicle without AS1411, nanovehicle with AS1411, and nanovehicle with AS1411 as well as endocytosis inhibitor. (C) Mean fluorescence intensity (MFI) of 4T1 cells from flow cytometry analysis in (B). (D) Confocal fluorescence images and (E) flow cytometry analysis of 4T1 cells after incubation with TOADI for 2, 4, and 6 h without laser irradiation and 6 h with laser irradiation (808 nm, 1.0 W/cm2, 5 min). (F) MFI of 4T1 cells from flow cytometry analysis in (E) (excitation wavelength: 480 nm for DOX, pseudocolor red; 780 nm for ICG, pseudocolor green; scale bar = 20 μm). The data are presented as the mean ± SD (n = 3). ***p < 0.001