Fig. 6

In vivo biodistribution and photothermal imaging. ICG was served as an imaging agent to create in vivo fluorescence imaging. (A) In vivo fluorescence images at 2, 4, 8, and 24 h and (B) Fluorescence photographs of ex vivo tissue and main organs of 4T1 tumor-bearing mice at 24 h post-injection after tail vein injection with free ICG, TODI, and TOADI. (Sp, Ki, He, Lu, Li, and Tu represent for spleen, kidney, heart, lung, liver, and tumor, respectively) (C) Semi-quantitative biodistribution of free ICG, TODI and TOADI in mice determined by the MFI of the tumor, respectively and semi-quantitative analysis (D) of tumors and major organs at 24 h post-injection. (E) Infrared thermal imaging and (F) temperature change curves of tumors from mice treated with PBS, TODI and TOADI under 808 nm laser irradiation. The data are presented as the mean ± SD (n = 3). **p < 0.01, ***p < 0.001