Fig. 2

The promotion effect of ADSC-Exos. a Confocal images of C2C12 cells incubated with 20 μg/mL PKH26-labeled ADSC-Exos (red) for 24 h (Scale bar: 50 μm). b Representative micrographs show that C2C12 cells were stained with Hoechst (blue) and EdU (green) (Scale bar: 50 μm). c Qualification analysis of the proliferation rate. The data were collected as the six randomly chosen fields from three independent experiments (n = 5). d Images of migrated C2C12 cells (Scale bar: 50 μm). e Quantitative analysis of the migration rate of C2C12 cells. The data are expressed as six randomly chosen fields from three independent experiments (n = 5). f HUVECs tube formation in a Matrigel assay (Scale bar, 100 μm). g Qualification of the closed tubular structure shown in f (n = 3). h Protein levels in C2C12 cells treated with 20 μg/mL ADSC-Exos. *p < 0.05, **p < 0.01, ****p < 0.0001