Fig. 4

ADSC-Exos prevented the inflammatory response and enhanced neovascularization in vivo. a Representative HE of gastrocnemius muscles derived from the ischemic hindlimb (Scale bar: 20 µm, red arrows point to inflammatory infiltration). b Quantification of the number of centronuclear myofibers within the ischemic muscle at days 14 and 21 (n = 5). c Representative Masson staining of gastrocnemius muscles derived from ischemic hindlimb (Scale bar: 100 µm). d Quantification of collagen deposition at days 14 and 21 after ischemic injury (n = 5). e Immunofluorescent staining of gastrocnemius muscles given the different treatments at day 14 post-ischemia. Smooth muscle cells (a-SMA), endothelial cells (CD34), and cell nuclei (DAPI) were stained with red, green, and blue colors (Scale bar, 100 μm). f Quantification of newly formed vessels stained with green color (n = 5). g Quantification of mature vessels stained with red colors (n = 5, scale bar, 100 μm). *p < 0.05, **p < 0.01, ***p < 0.001