Fig. 7

miR-125b-5p directly regulated the expression of ACER2 in C2C12 cells. a Venn diagram representing the number of predicted targets of miR-125b-5p. b Schematic of the putative binding sites or mutations of miR-125b-5p in ACER2 mRNA 3’UTR. c Luciferase reporter assay determined ACER2 as the target of miR-125b-5p (n = 3). d The mRNA expression of ACER2 in each group (n = 4). e, f Western blotting analysis of ACER2 protein expression in C2C12 cells. **p < 0.01, ***p < 0.001