Fig. 4
In vitro mild hyperthermia-starving mutual synergistic therapy against 143B cells. (A) Flow cytometry was used to determine and analyze the apoptosis of 143B cells cocultured with PBS, AMF, GOx, Fe3O4/MgCO3@PLGA, and MBRs for 24 and 48 h. (B) Cell viability assay and (C) flow cytometry analysis of 143B cells incubated with PBS, Fe3O4/MgCO3@PLGA and MBRs after AMF exposure for 24 and 48 h. (D) Cell viability assay results of 143B cells after various cycles of “on-off” model magnetothermal treatment for Fe3O4/MgCO3@PLGA and MBRs groups. (E) Calcein-AM (green)/PI (red) staining images of 143B cells after various treatments (scale bars: 200 μm). (The data are shown as the means ± SDs, n = 3 per group, n.s. represents no significance and #p < 0.05, ##p < 0.01, in comparison with the control groups, *p < 0.05 **p < 0.01 in comparison between groups, respectively.)