Fig. 6
Evaluation of the MBRs ability for bone regeneration in vitro and in vivo. (BA) ALP staining and alizarin red staining (ARS) of PBS, PLGA, Fe3O4/MgCO3@PLGA and MBRs. Quantitative analysis of (B) ALP activity and (C) ARS staining. (D) Reconstructed 3D micro-CT images of rat crania with the treated defects (left: self-blank control, right: treatment with different groups). (E) Histological evaluation of bone defect regeneration by H&E, safranin O-fast green and Masson’s trichrome staining (the repair area is labeled with rectangular boxes, scale bars: 100 μm). Quantitative analysis of bone defect regeneration as determined by (F) the defect coverage percent, (G) the score of bone defect restoration, (H) BV/TV% and (I) BMD: (a) within the MBRs group and (b) among the three groups. (The data are shown as the means ± SDs, n = 3 per group, n.s. represented no significance and *p < 0.05, **p < 0.01 in comparison with the control groups, # p < 0.05 ## p < 0.01 in comparison with PLGA groups, respectively