Fig. 6

Co-efficient analysis of neuritic plaques stained with Congo Red and labelled PLGA in the cortex. A-G; Image co-localization analysis demonstrated by scatter plots (A-F) and Pearson’s co-efficient-Rr values (G) for the merged images of native PLGA labelled amyloid plaques in 5xFAD brain cortical tissues as a function of time. In the scatter plot, green signal intensity of native PLGA is represented on the y-axis and red pixel intensity of Congo Red labelled plaques is represented on the x-axis. A Pearson’s co-efficient-Rr value of 1 represents perfect colocalization, while 0 represents no colocalization. Note the level of colocalization as a function of time (A-F) reflected in the Pearson’s co-efficient graph with a max peak Rr value of 0.67 ± 0.05 at 3 h (G). Differences in the mean Rr values at 1 week compared to other time points (1 h, 3 h, 12 h, 24 and 72 h) were assessed by one-way ANOVA employing Dunnett’s multiple comparisons post-hoc tests. * p < 0.05; **p < 0.01 and ***p < 0.001 H; Graph representing integrated fluorescence densities of labelled PLGA (lavender) and Congo Red labelled plaques (blue) from 5xFAD mouse brain images depicting a decline in fluorescence densities of PLGA, but not Congo Red, as a function of time over 72hr period