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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Metal-organic framework-encapsulated dihydroartemisinin nanoparticles induces apoptotic cell death in ovarian cancer by blocking ROMO1-mediated ROS production

Fig. 6

The cytotoxic effects of ZIF-DHA against ovarian cancer cells depended on ROMO1. (A) Treatment with ZIF-DHA reduced ROMO1 and its downstream signaling pathways. Ovarian cancer cells SKOV3 and A2780 were treated with free ZIF, free DHA or ZIF-DHA. Cell lysates were then blotted with indicated antibodies. (B) Ovarian cancer cells SKOV3 and A2780 cells stably expressing vector or Flag-ROMO1 were treated with ZIF-DHA. Cell lysates were then blotted with indicated antibodies. (C-E) The DCFDA/H2DCFDA Kit was used to explore the overexpression of ROMO1 in regulation of ZIF-DHA-induced ROS generation. (F-H) The Annexin V-FITC Apoptosis Detection Kit was used to explore the overexpression of ROMO1 in regulation of ZIF-DHA-induced apoptosis. (I-K) The colony formation assay was used to explore the overexpression of ROMO1 in regulation of ZIF-DHA-induced growth inhibition

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Journal of Nanobiotechnology

ISSN: 1477-3155

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