Fig. 3

A Cumulative transport of poly(MIs)/PTX@PEI/siPGK1@CCM at different time points across the bEnd.3 monolayer. Inset: schematic illustration of BBB model. B CLSM images of U87 cells incubated with commercial Lipofectamine 2000 and poly(MIs)/PTX@PEI/siPGK1@CCM for different times. Green fluorescence shows location of the FAM-siPGK1. The lysosomes/endosomes were stained with LysoTracker Red (red), and the cell nuclei were stained with DAPI (blue). C The cellular uptake and release behavior after incubation for 4 h in hypoxic and normoxic conditions. D The percentages of each cell cycle subpopulation in poly(MIs)/PTX@PEI/siPGK1@CCM-treated U87 cells. E PGK1 protein inhibition capacity after 24 h of transportation