Fig. 4

A Live/dead cell staining results of U87 cells costained with calcein-AM (live cells, green) and PI (dead cells, red) after hypoxia-responsive activated poly(MIs)/PTX@PEI/siPGK1@CCM treatment at various concentrations. B Representative images of the clonogenic survival assays of U87 cells cultured with different formulated micelles under hypoxic conditions (pO₂: 2%) following treatment with 0, 2, 4, 6, or 8 Gy. C EdU-positive cells indicating actively dividing cells. D Apoptosis assay of U87MG cells after different treatments and were analyzed using flow cytometry