Fig. 5

A Real-time fluorescence imaging of mice treated with (1) poly(MIs)/PTX@PEI/siPGK1@CCM, (2) poly(MIs)/PTX@PEI/siPGK1@LipoPEG and (3) Free siPGK1, after 20 days of tumor cell inoculation. B Ex vivo fluorescence images of major organs dissected from mice injected with poly(MIs)/PTX@PEI/siPGK1@CCM, poly(MIs)/PTX@PEI/siPGK1@LipoPEG and Free siPGK1 (2.3 mg siRNA equiv/kg) at 8 h post injection. H, Li, Sp, Lu, Ki, and Br represent heart, liver, spleen, lung, kidney, and brain, respectively. (C) Blood retention kinetics of the free siPGK1, poly(MIs)/PTX@PEI/siPGK1@LipoPEG and poly(MIs)/PTX@PEI/siPGK1@CCM in tumor-free BALB/C mice. D Quantitative analysis of siPGK1 in brain tissues at different time points. E Quantitative biodistribution of the different treatments in the brains and major organs of the mice at 0 h, 2 h, 4 h, 8 h, 12 h and 24 h. Data are shown as mean ± SD (n = 3). (*p < 0.05, **p < 0.01, ***p < 0.001)