Fig. 6

A Schematic protocol of animal experiments. B In vivo efficacy in the orthotopic U87MG-luc mouse model by bioluminescence signal (saline, saline + RT, free PTX + naked siPGK1 + RT, poly(MIs)/PTX@PEI/siPGK1@CCM (without RT), poly(MIs)@PEI/siPGK1@CCM + RT, poly(MIs)/PTX@PEI@CCM + RT, poly(MIs)/PTX@PEI/siPGK1@CCM + RT at doses of 1.8 mg/kg PTX and 2.3 mg/kg siPGK1) on days 10, 20 and 30 with 2 Gy RT. C Typical H&E staining images of tumors (coronal staining). D Kaplan–Meier survival curves for the mice (n = 10) after different treatment strategies. E Body weight change in mice. The data are presented as the mean ± SDs. F Tumor slices excised from nude mice bearing orthotopic U87MG-luc human glioblastoma tumors following treatment using PGK1, rH2AX, TUNEL and proliferation (Ki67) staining