Fig. 3
In vitro tumor cell uptake and cytotoxicity. (A) CDDP loading capacities of Her2-ANs. (B) Release profiles of Her2-ANs@CDDP at different pH values and different GSH concentrations. (C) Intracellular uptake of ANs and Her2-ANs (labeled with FITC) in the U2OS-Her2(+) cells was measured by confocal laser scanning microscopy. Scale bar: 100 μm. (D) Intracellular uptake of ANs and Her2-ANs (labeled with FITC) in the U2OS-Her2(+) cells was measured by flow cytometry. (E) Statistical analyses of the intracellular uptake rate of ANs and Her2-ANs. (F, G) Cytotoxicity of ANs and Her2-ANs against U2OS-Her2(+) cells (F). Relative viabilities of U2OS-Her2(+) and U2OS-Her2(-) cells after incubation with various treatments (G) (CDDP: 2 µg/mL; As ions: 10 µg/mL). (H, I) Cytotoxicity of ANs and Her2-ANs against 143B-Her2(+) cells (H). Relative viabilities of 143B-Her2(+) and 143B-Her2(-) cells after incubation with various treatments (I) (CDDP: 1 µg/mL; As ions: 10 µg/mL). (Values are presented as means ± sd, n = 3, *P < 0.05, **P < 0.01.)