Fig. 4
Mechanism evaluation of ANs induced cell death by ferroptosis. (A, B) Flow cytometry analysis of the production of ROS (A) and lipid peroxide (B) in U2OS-Her2(+) cells after different treatments. (C, D) Confocal laser scanning microscopy imaging of the production of ROS (C) and lipid peroxide (D) levels in U2OS-Her2(+) cells after different treatments. Scale bar: 100 μm. (E, F) GSH: GSSG ratio (E) and GPX4 activity (F) in U2OS-Her2(+) cells treated with Her2-ANs. (G) Western blot results for GPX4 expression in U2OS-Her2(+) cells after treatment with Her2-ANs. (H) Schematic illustration of Her2-ANs induced ferroptosis. Arsenene nanosheets could effectively deplete intracellular GSH and then induce ferroptosis by inhibiting GPX4. (I, J) TEM (I) and SEM (J) reveal nuclear, mitochondrial, and cell membrane alterations in U2OS-Her2(+) cells after treatment with Her2-ANs. (The white arrow represents DNA damage, the red arrow represents mitochondria, and the blue arrow represents cell membrane damage.)