Fig. 4

Oral EUP-SeNP improved DSS-induced intestinal barrier function. A AB-PAS staining of colon; the scale bar is 50 μm. B Mucin (MUC2) was stained by immunohistochemistry, the scale bar is 50 μm. C IOD was counted of MUC2 by immunohistochemical staining; D Protein bands analysis of colonic tight-junction proteins Occludin, claudin-1, claudin-3 and ZO-1; E–H Protein statistical analysis of colonic tight-junction proteins Occludin, claudin-1, claudin-3 and ZO-1. Data are means ± SEM by one-way ANOVA with Bonferroni’s multiple comparisons test. “*”P < 0.05, “**”P < 0.01, “***”P < 0.001 and “****”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “#”P < 0.05, “##”P < 0.01, “###”P < 0.001 and “####”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “*” represents the significance between the control group and the DSS model group; “#” represents the significance between the DSS model group and the treatment group