Fig. 5

Oral EUP-SeNP alleviated DSS-induced colitis by improving level of apoptosis, proliferation and inflammatory. A TUNEL staining of colon, the scale bar is 50 μm. B Ki67 was stained by immunohistochemistry, the scale bar is 50 μm. C IOD was counted of TUNEL by Immunofluorescence staining; D IOD was counted of Ki67 by immunohistochemical staining. E–J The expression of IL-1β, IL-6, IL-10, IL-12, IL-17 and TNF-α proteins in colon was detected by ELISA. Data are means ± SEM by one-way ANOVA with Bonferroni’s multiple comparisons test. “*”P < 0.05, “**”P < 0.01, “***”P < 0.001 and “****”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “#”P < 0.05, “##”P < 0.01, “###”P < 0.001 and “####”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “*” represents the significance between the control group and the DSS model group; “#” represents the significance between the DSS model group and the treatment group