Fig. 9

EUP-SeNP can protect against LPS-induced cell injury by enhancing intestinal barrier function and regulating NF-KB signaling pathway. A Protein bands analysis of IEC-6; B Protein bands analysis of Caco-2; E–G Protein statistical analysis of claudin-1, ZO-1, pIKB/IKB, pp65/p65 and TRL-4 of IEC-6. H, I Protein statistical analysis of claudin-1,ZO-1, pIKB/IKB, pp65/p65 and TRL-4 of Caco-2. M TUNEL staining of IEC-6, the scale bar is 50 μm. N TUNEL staining of Caco-2, the scale bar is 50 μm. IEC-6 and Caco-2 were induced by LPS for 36 h, and the Pre and Post groups were treated with 10ug/mL EUP-SeNP for 12 h. O IOD of TUNEL staining ofEC-6. P IOD of TUNEL staining of Caco-2. Q mRNA expression of inflammatory cytokines IL-17,IL-1β, IL-10,TNF-α, and IL-6 in IEC-6. Data are means ± SEM by one-way ANOVA with Bonferroni’s multiple comparisons test. “*”P < 0.05, “**”P < 0.01, “***”P < 0.001 and “****”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “#”P < 0.05, “##”P < 0.01, “###”P < 0.001 and “####”P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons test. “*” represents the significance between the control group and the DSS model group; “#” represents the significance between the DSS model group and the treatment group