Fig. 4

Differential centrifugation for ApoBDs isolation. After induction of apoptosis by UV or staurosporine, the supernatant is centrifuged at 300–400g for 10 min at 4 ℃ to remove the large apoptotic cells and fragments. Then, the supernatant is centrifuged at 1000g–4000g for 15–30 min at 4 ℃ to separate micron-size ApoBDs. At last, nano-size ApoBDs are obtained from precipitate through centrifugation of the supernatant at 16,000g for 30 min at 4 ℃. Created with BioRender.com