Fig. 2
Cellular uptake of Bi2S3-3BP and inhibition of glycolysis. (a-b) Intracellular uptake of Bi2S3-3BP (labeled with FITC) observed by CLSM and quantified by flow cytometry analysis. (c) HK-II and GAPDH expression measured by western blot. (d-e) Quantitative analysis of relative HK-II and GAPDH expression (n = 3). (f) Intracellular lactate levels (n = 3). (g) Intracellular ATP levels (n = 3). (h) Schematic illustration about measuring the O2 consumption. (i) Relative dissolved oxygen (D.O.) changes in the cell media of different groups (n = 3). (j-k) Cells stained with ROS-ID in different groups observed by CLSM and quantified by flow cytometry. n.s.: no significance, *P < 0.05, **P < 0.01, ***P < 0.001