Fig. 4
Pro-death autophagy of Bi2S3-3BP. (a) LC3-II, LC3-I, and P62 expression after different treatments as measured by western blot. (b-c) Quantitative analysis of relative LC3-II/LC3-1 and P62 expression. (d) Representative immunofluorescence images of LC3 punctate dots and MDC in different groups. (e) TEM images showing the formation of autophagosomes after different treatments. Zoomed-in TEM images in typical structures of autophagosomes are indicated with arrows. (f-g) Cell viability of 4T1 cells after being treated with Bi2S3 + X-ray or Bi2S3-3BP + X-ray in the presence of RAPA or 3-MA (n = 5). n.s.: no significance, *P < 0.05, **P < 0.01, ***P < 0.001