Fig. 5

In vivo hypoxia relief, ROS generation and autophagy. (a) PA images of tumor sites in oxy-hemoglobin mode at different time points. (b) Quantification of oxyhemoglobin saturation at tumor sites (n = 3). (c-d) Immunochemical staining of HIF-1α and pimonidazole on tumor sections from 4T1 tumor-bearing mice after various treatments. (e-f) Mean fluorescence intensity semiquantitative analysis of HIF-1α and pimonidazole (n = 5). (g-j) Immunochemical staining of ROS and LC3 on tumor sections from 4T1 tumor-bearing mice, and the corresponding semiquantitative analysis (n = 5). n.s.: no significance, *P < 0.05, **P < 0.01, ***P < 0.001