Fig. 6
From: Extracellular vesicles: a rising star for therapeutics and drug delivery
Reprinted with permission from Ref [182]
a Confocal imaging of DOX and NEs-Exos/DOX crossing the zebrafish BBB at 30 min. DOX and NEs-Exos/DOX were intracardially injected into the circulation of Tg (fli:GFP) zebrafish. b Immunofluorescence staining of IBA1 (green) and GFAP (magenta) in brain tissue. c In vivo distribution of PKH26-labeled NEs-Exos (red) in brain with LPS-induced brain inflammation. Brain treated with saline was considered as the control. Immunofluorescence of IBA1 (marker of microglia activation, green) and GFAP (marker of astrocytes, yellow) indicate the inflammatory condition after LPS and saline treatment. d Real-time fluorescence tracking of DiR and DiR-labeled NEs-Exos in C6-Luc glioma-bearing mice. e Body weight changes and Kaplan-Meier survival analysis of glioma-bearing nude mice. *P < 0.05, **P < 0.01 and ****P < 0.0001 indicate significant differences.