Fig. 12
From: Next-generation nanomaterials: advancing ocular anti-inflammatory drug therapy
Reproduced from Ref. [346] with permission from Springer Nature
The pivotal roles of Pax6, Bmi1, and Ki67 in ocular development and lens-induced regeneration. A This panel illustrates the role of Pax6 in eye and lens development. In progeny derived from crossing the ROSAmTmG membrane-bound GFP reporter strain with (P0-3.9-GFPcre) mice, robust GFP expression was noted in lens epithelial cells (LECs) under fluorescence microscopy, indicating that Pax6 LECs from embryonic or adult lenses contribute to the regeneration of mouse lens fiber cells. B The part shows that deletion of Bmi-1 results in diminished Pax6 + and Sox2 + expression in LECs. The vertical axis represents the percentage of Pax6 + and SOX2 + cells. Data are presented as mean ± SD; statistical significance is denoted by *P < 0.001. The part C reveals that the absence of Bmi1 leads to a decrease in LEC proliferation. The proportion of BrdU + LECs was calculated for each eye at 2 m, 7 m, and 12 m. Statistical significance was determined via a two-tailed Student’s t-test; *P < 0.05. Part D displays Nestin (green) staining images of wild-type mice at E13.5, E18.5, and 2 months of age, as observed through fluorescence microscopy. Part E depicts the histological examination (HE staining) of eyeballs from 2 m, 7 m, and 12-month-old Bmi1fl/fl control mice and Nestin-cre; Bmi1fl/fl mice, to observe the development of cataracts. After using Nestn-creER to delete Bmi-1 in 6-week-old mice and following 10 months of tamoxifen treatment, the HE morphology of mouse eyes showed no cataract phenotype. All scale bars equal 100 μm.