Fig. 7
From: Next-generation nanomaterials: advancing ocular anti-inflammatory drug therapy
The use of Tacrolimus-loaded mPEG-b-PLGA micelles in the treatment of corneal immune rejection subsequent to allogeneic penetrating corneal transplantation in rats A. (a) presents a schematic diagram of the composition and ocular application of Tacrolimus-loaded mPEG-b-PLGA micelles, while (b) shows the detection of mPEG-b-PLGA by a 1H NMR spectrometer. (c) provides a scanning electron microscope (SEM) image of Tacrolimus-loaded mPEG-b-PLGA micelles with a scale bar denoting 50 nm. The immunofluorescence assay was used to observe the inhibitory effect of Tacrolimus-loaded mPEG-b-PLGA micelles (administered either via subconjunctival injection, or solution drops) on the phosphorylation of NFAT B, CD4 C and CD8 D, key factors in immune rejection, across various layers of corneal tissue in comparison to blank controls and standard 0.05% tacrolimus eye drops. E showcases a statistical graph of the percentage of fluorescence intensity. Values are represented as mean ± SD, with statistical significance denoted as *p < 0.05, **p < 0.01, ***p < 0.001. This figure is reproduced from Ref. [249] with permission from Elsevier