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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: CAFs targeted ultrasound-responsive nanodroplets loaded V9302 and GLULsiRNA to inhibit melanoma growth via glutamine metabolic reprogramming and tumor microenvironment remodeling

Fig. 3

Characterization of FH-V9302-siGLUL-NDs and gene silencing efficiency. (A) Agarose gel electrophoresis retardation assay of FH-V9302-NDs with siGLUL in different volume ratios. (B) ζ potential changes of siGLUL with FH-V9302-NDs at different volume ratios (n = 3). (C) ζ potential of FH-V9302-NDs and FH-V9302-siGLUL-NDs (n = 3). (D) Western blot analysis of B16F10 and CAFs cells with different treatment knockdown GLUL levels. (E) Western blot analysis of different nanodroplets loading siGLUL knockdown GLUL levels. (F&G) Quantitative analysis of GLUL expression in B16F10(F) and CAFs(G) cells under different treatments. (H&I) Quantitative analysis of GLUL expression in B16F10 (H) and CAFs (I) cells treated with different nanodroplets loading siGLUL. *p < 0.05, **p < 0.01, ****p < 0.0001 (ANOVA test). All statistical data are expressed as means ± SD (n = 3)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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