Fig. 8

Anti-tumor ability in vitro. (A) Fluorescence images of EdU-positive cells after 24 h of various treatments. Scale bar: 100 μm. (B&C) Treated B16F10 cells were seeded on the upper chamber with or without matrigel. After 24 h invaded cells were detected and images captured and analyzed as described in Materials and Methods. Image of migration (B) and invasion (C) cells examined by Transwell assay. Scale bar: 200 μm. (D) Quantification of the percentage of EdU-positive cells. (E) Cell viability of B16F10 cells after 24 h of various treatments. (F&G) Quantification of the migrating cells (F) and invading cells(G). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001(ANOVA test). All statistical data are expressed as means ± SD (n = 3)