Fig. 8

Assessment of myocardial fibrosis by pathological staining and western blot at day 28 post the treatment. (A) Masson trichrome staining at the level of cardiac papillary muscle, the semi-quantitative analysis of LV wall thickness (C) and collagen volume fraction (D), In Masson staining, collagen was stained blue, scale bars of heart cross sections of Masson trichrome staining: 2.5 mm. (B) Sirius Red staining (Sirius red staining was observed at 40 magnification, and scale bars is 0.5 mm. The red staining was collagen, the red and yellow tissues observed were collagen I, while the green tissues observed was collagen III). (E) The semi-quantitative analysis of collagen I/III ratio based on Sirus red staining. (F) the western blot of collagen I and III in myocardial infarction tissue and the semi-quantitative analysis (G&H). All bars represent as means ± SD (n = 3). *P < 0.05 and **P < 0.01, ***P < 0.001