Table 1 Primer sequences used in quantitative real-time PCR
Gene | Primers |
|---|---|
GAPDH (homo) | Forward: 5′-ACCCACTCCTCCACCTTTGAC-3′ Reverse: 5′-TCCACCACCCTGTTGCTGTAG-3′ |
RUNX2 (homo) | Forward: 5′-ACCAGCAGCACTCCATATCTCTAC-3′ Reverse: 5′-CTTCCATCAGCGTCAACACCATC-3′ |
ALPL (homo) | Forward: 5′-ACTGGTACTCAGACAACGAGAT-3′ Reverse: 5′-ACGTCAATGTCCCTGATGTTATG-3′ |
COL1A1 (homo) | Forward: 5′-TCGGAGGAGAGTCAGGAAGG-3′ Reverse: 5′-TCAGCAACACAGTTACACAAGG-3′ |
GAPDH (mus) | Forward: 5’-AGGTGGTGAAGCAGGCATC − 3’ Reverse: 5’-AAGGTGGAAGAGTGGGAGTTG − 3’ |
IL-1β (mus) | Forward: 5’-CCAAGCAATACCCAAAGAAGAAGATG-3’ Reverse: 5’-TTATGTCCTGACCACTGTTGTTTCC-3’ |
IL-6 (mus) | Forward: 5’-GAAACCGCTATGAAGTTCCTCTCT-3’ Reverse: 5’-GTATCCTCTGTGAAGTCTCCTCTCC-3’ |
IL-10 (mus) | Forward: 5’-CCCCAGCCGCTTCATCCC-3’ Reverse: 5’-ACAAACAATACACCATTCCCAGAGG-3’ |
TNF-α (mus) | Forward: 5’-AAGGGAGAGTGGTCAGGTTGC-3’ Reverse: 5’-TGGAAAGGTCTGAAGGTAGGAAGG-3’ |
iNOS (mus) | Forward: 5’-GCACCACCCTCCTCGTTCAG-3’ Reverse: 5’-CCACAACTCGCTCCAAGATTCC-3’ |
ARG1 (mus) | Forward: 5’- GCCTTTGTTGATGTCCCTAATG-3’ Reverse: 5’- GCACCACACTGACTCTTCC-3’ |